Semaphorin3B promotes an anti-inflammatory and pro-resolving phenotype in macrophages from rheumatoid arthritis patients in a MerTK-dependent manner.

ABSTRACT: Previous works from our group show that Semaphorin3B (Sema3B) is reduced in RA and plays a protective role in a mouse arthritis model. In turn, MerTK plays a protective function in murine arthritis models, is expressed by synovial tissue macrophages and is linked to remission in patients with RA. In this study, we examined the role of Sema3B in the phenotypic characteristics of RA macrophages and the implication of MerTK. Peripheral blood monocytes from RA patients were differentiated into IFN-γ (RA MØ_IFN-γ) or M-CSF (RA MØ_M-CSF) macrophages and stimulated with LPS, Sema3B or their combination. Alternatively, RA fibroblast like synoviocytes (FLS) were stimulated with RA MØ_IFN-γ and RA MØ_M-CSF supernatants. Gene expression was determined by qPCR a

SUBMITTER: Martinez-Ramos S 

PROVIDER: S-EPMC10811190 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature