Production and secretion of recombinant spider silk in Bacillus megaterium.

ABSTRACT:

Background

Silk proteins have emerged as versatile biomaterials with unique chemical and physical properties, making them appealing for various applications. Among them, spider silk, known for its exceptional mechanical strength, has attracted considerable attention. Recombinant production of spider silk represents the most promising route towards its scaled production; however, challenges persist within the upstream optimization of host organisms, including toxicity and low yields. The high cost of downstream cell lysis and protein purification is an additional barrier preventing the widespread production and use of spider silk proteins. Gram-positive bacteria represent an attractive, but underexplored, microbial chassis that may enable a reduction in the cost and difficulty of re

SUBMITTER: Connor A 

PROVIDER: S-EPMC10821235 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature