Protease-activated receptors and glycoprotein VI cooperatively drive the platelet component in thromboelastography.

ABSTRACT:

Background

Thromboelastography (TEG) is used for real-time determination of hemostatic status in patients with acute risk of bleeding. Thrombin is thought to drive clotting in TEG through generation of polymerized fibrin and activation of platelets through protease-activated receptors (PARs). However, the specific role of platelet agonist receptors and signaling in TEG has not been reported.

Objectives

Here, we investigated the specific receptors and signaling pathways required for platelet function in TEG using genetic and pharmacologic inhibition of platelet proteins in mouse and human blood samples.

Methods

Clotting parameters (R time, α-angle [α], and maximum amplitude [MA]), were determined in recalcified, kaolin-triggered citrated blood samples using a TEG 5000

SUBMITTER: Rudran T 

PROVIDER: S-EPMC10824270 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature