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Widespread Peptide Surfactants with Post-translational C-methylations Promote Bacterial Development.

ABSTRACT: Bacteria produce a variety of peptides to mediate nutrient acquisition, microbial interactions, and other physiological processes. Of special interest are surface-active peptides that aid in growth and development. Herein, we report the structure and characterization of clavusporins, unusual and hydrophobic ribosomal peptides with multiple C-methylations at unactivated carbon centers, which help drastically reduce the surface tension of water and thereby aid in Streptomyces development. The peptides are synthesized by a previously uncharacterized protein superfamily, termed DUF5825, in conjunction with a vitamin B₁₂-dependent radical S-adenosylmethionine metalloenzyme. The operon encoding clavusporin is wide-spread among actinomycete bacteria, suggesting a prevalent role for clavusporins as morphogens in erecting aerial hyphae and thereby advancing sporulation and proliferation.

SUBMITTER: Zhang C

PROVIDER: S-EPMC10849626 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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Widespread Peptide Surfactants with Post-translational C-methylations Promote Bacterial Development.

Zhang Chen C Seyedsayamdost Mohammad R MR

bioRxiv : the preprint server for biology 20240124

Bacteria produce a variety of peptides to mediate nutrient acquisition, microbial interactions, and other physiological processes. Of special interest are surface-active peptides that aid in growth and development. Herein, we report the structure and characterization of clavusporins, unusual and hydrophobic ribosomal peptides with multiple C-methylations at unactivated carbon centers, which help drastically reduce the surface tension of water and thereby aid in Streptomyces develop ...[more]

PMID: 38328144

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