Ultrasensitive, Multiplexed Buoyant Sensor for Monitoring Cytokines in Biofluids.

ABSTRACT: Multiplexed quantification of low-abundance protein biomarkers in complex biofluids is important for biomedical research and clinical diagnostics. However, in situ sampling without perturbing biological systems remains challenging. In this work, we report a buoyant biosensor that enables in situ monitoring of protein analytes at attomolar concentrations with a 15 min temporal resolution. The buoyant biosensor implemented with fluorescent nanolabels enabled the ultrasensitive and multiplexed detection and quantification of cytokines. Implementing the biosensor in a digital manner (i.e., counting the individual nanolabels) further improves the low detection limit. We demonstrate that the biosensor enables the detection and quantification of the time-varying concentrations of cytokines (e.g.,

SUBMITTER: Guo H 

PROVIDER: S-EPMC10863391 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature