Algal rhodopsins encoding diverse signal sequence holds potential for expansion of organelle optogenetics.

ABSTRACT: Rhodopsins have been extensively employed for optogenetic regulation of bioelectrical activity of excitable cells and other cellular processes across biological systems. Various strategies have been adopted to attune the cellular processes at the desired subcellular compartment (plasma membrane, endoplasmic reticulum, Golgi, mitochondria, lysosome) within the cell. These strategies include-adding signal sequences, tethering peptides, specific interaction sites, or mRNA elements at different sites in the optogenetic proteins for plasma membrane integration and subcellular targeting. However, a single approach for organelle optogenetics was not suitable for the relevant optogenetic proteins and often led to the poor expression, mislocalization, or altered physical and functional properties.

SUBMITTER: Sushmita K 

PROVIDER: S-EPMC10865886 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature