Protocol for the isolation of GFP-expressing ferroptosis-dependent extracellular vesicles in in vitro cell culture models.
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SUBMITTER: Ito F
PROVIDER: S-EPMC10877193 | biostudies-literature | 2024 Feb
REPOSITORIES: biostudies-literature
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Protocol for the isolation of GFP-expressing ferroptosis-dependent extracellular vesicles in in vitro cell culture models.
Ito Fumiya F Yanatori Izumi I Kato Katsuhiro K Toyokuni Shinya S
STAR protocols 20240215 1
Extracellular vesicles (EVs) are complex structures that transport various DNA, RNA, and protein. Recently, new EV secretion mechanisms have been identified through the iron regulatory system in mammalian cells. We revealed that ferroptosis increases EV secretion, which is named ferroptosis-dependent EVs (FedEVs). Here, we describe a step-by-step procedure to isolate GFP-expressing FedEVs for in vitro analysis. The FedEVs are further analyzed by imaging and flow cytometry analysis. For complete ...[more]
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