Dynamic hydrogen peroxide levels reveal a rate-dependent sensitivity in B-cell lymphoma signaling.

ABSTRACT: Although in vivo extracellular microenvironments are dynamic, most in vitro studies are conducted under static conditions. Here, we exposed diffuse large B-cell lymphoma (DLBCL) cells to gradient increases in the concentration of hydrogen peroxide (H₂O₂), thereby capturing some of the dynamics of the tumour microenvironment. Subsequently, we measured the phosphorylation response of B-cell receptor (BCR) signalling proteins CD79a, SYK and PLCγ2 at a high temporal resolution via single-cell phospho-specific flow cytometry. We demonstrated that the cells respond bimodally to static extracellular H₂O₂, where the percentage of cells that respond is mainly determined by the concentration. Computational analysis revealed that the bimodality results from

SUBMITTER: Witmond M 

PROVIDER: S-EPMC10882005 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature