The hypothalamic-pituitary-thyroid axis is intact in male insulin receptor substrate 4 knockout mice.

ABSTRACT:

Objective

Loss of function mutations in the insulin receptor substrate 4 (IRS4) gene cause a rare form of X-linked congenital central hypothyroidism in boys and men. Affected individuals show decreased thyroid-stimulation hormone (TSH) secretion. Members of the IRS family canonically act as scaffold proteins between tyrosine kinase receptors and downstream effectors. How loss of IRS4 affects TSH synthesis or secretion is unresolved. We therefore assessed IRS4's role in the hypothalamic-pituitary-thyroid axis of Irs4 knockout mice.

Methods

We generated two global Irs4 knockout mouse lines harboring either two or four base-pair deletions that result in frameshifts and loss of most of the IRS4 protein.

Results

Under normal laboratory conditions, Irs4 knockout males did n

SUBMITTER: Brule E 

PROVIDER: S-EPMC10895334 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature