Single-cell analyses of polyclonal Plasmodium vivax infections and their consequences on parasite transmission.

ABSTRACT: Most Plasmodium vivax infections contain genetically distinct parasites, but the consequences of this polyclonality on the development of asexual parasites, their sexual differentiation, and their transmission remain unknown. We describe infections of Saimiri monkeys with two strains of P. vivax and the analyses of 117,350 parasites characterized by single cell RNA sequencing and individually genotyped. In our model, consecutive inoculations fail to establish polyclonal infections. By contrast, simultaneous inoculations of two strains lead to sustained polyclonal infections, although without detectable differences in parasite regulation or sexual commitment. Analyses of sporozoites dissected from mosquitoes fed on coinfected monkeys show that all genotypes are successf

SUBMITTER: Hazzard B 

PROVIDER: S-EPMC10896380 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature