Simultaneous proteome localization and turnover analysis reveals spatiotemporal features of protein homeostasis disruptions.

ABSTRACT: The spatial and temporal distributions of proteins are critical to protein function, but cannot be directly assessed by measuring protein bundance. Here we describe a mass spectrometry-based proteomics strategy, Simultaneous Proteome Localization and Turnover (SPLAT), to measure concurrently protein turnover rates and subcellular localization in the same experiment. Applying the method, we find that unfolded protein response (UPR) has different effects on protein turnover dependent on their subcellular location in human AC16 cells, with proteome-wide slowdown but acceleration among stress response proteins in the ER and Golgi. In parallel, UPR triggers broad differential localization of proteins including RNA-binding proteins and amino acid transporters. Moreover, we observe newly synthesi

SUBMITTER: Currie J 

PROVIDER: S-EPMC10928085 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature