The mechanism of PFK-1 in the occurrence and development of bladder cancer by regulating ZEB1 lactylation.

ABSTRACT:

Background

Bladder cancer (BC) is one of the most common malignancies of the genitourinary system. Phosphofructokinase 1 (PFK-1) is one of member of PFK, which plays an important role in reprogramming cancer metabolism, such as lactylation modification. Zinc finger E-box-binding homeobox 1 (ZEB1) has been demonstrated to be a oncogene in many cancers. Therefore, this study was performed to explore the effects of PFK-1 on the lactylation of ZEB1 in BC development.

Methods

Cell viability was measured using the CCK-8 kit. The glucose assay kit and lactate assay kit were used to detect glucose utilization and lactate production. The DNA was purified and quantified by qRT-PCR.

Results

In the present study, we found that ZEB1 expression levels were significantly elevated in

SUBMITTER: Wang R 

PROVIDER: S-EPMC10935987 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature