Dataset Information

Double-blinded, randomized clinical trial of Gegen Qinlian decoction pinpoints Faecalibacterium as key gut bacteria in alleviating hyperglycemia.

ABSTRACT:

Background

Accumulating evidence suggests that metabolic disorders, including type 2 diabetes mellitus (T2DM), can be treated with traditional Chinese medicine formulas, such as the Gegen Qinlian decoction (GQD). This study elucidates the mechanisms by which gut microbes mediate the anti-diabetic effects of GQD.

Methods

We conducted a double-blind randomized clinical trial involving 120 untreated participants with T2DM. During the 12-week intervention, anthropometric measurements and diabetic traits were recorded every 4 weeks. Fecal microbiota and serum metabolites were measured before and after the intervention using 16S rDNA sequencing, liquid chromatography-mass spectrometry, and Bio-Plex panels.

Results

Anti-diabetic effects were observed in the GQD group in the human trial. Specifically, glycated hemoglobin, fasting plasma glucose, and two-hour postprandial blood glucose levels were significantly lower in the GQD group than in the placebo group. Additionally, Faecalibacterium was significantly enriched in the GQD group, and the short-chain fatty acid levels were higher and the serum inflammation-associated marker levels were lower in the GQD group compared to the placebo group. Moreover, Faecalibacterium abundance negatively correlated with the levels of serum hemoglobin, fasting plasma glucose, and pro-inflammatory cytokines. Finally, the diabetes-alleviating effect of Faecalibacterium was confirmed by oral administration of Faecalibacterium prausnitzii (DSMZ 17677) in T2DM mouse model.

Conclusions

GQD improved type 2 diabetes primarily by modulating the abundance of Faecalibacterium in the gut microbiota, alleviating metabolic disorders and the inflammatory state.

Trial registration

Registry No. ChiCTR-IOR-15006626.

SUBMITTER: Gao Z

PROVIDER: S-EPMC10941319 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Double-blinded, randomized clinical trial of Gegen Qinlian decoction pinpoints <i>Faecalibacterium</i> as key gut bacteria in alleviating hyperglycemia.

Gao Zezheng Z Zhang Wenhui W He Lisha L Wang Han H Li Yufei Y Jiang Xiaotian X D I Sha S Wang Xinmiao X Zhang Xuan X Han Lin L Liu Yanwen Y Gu Chengjuan C Wu Mengyi M He Xinhui X Cheng Lei L Wang Jun J Tong Xiaolin X Zhao Linhua L

Precision clinical medicine 20240214 1

<h4>Background</h4>Accumulating evidence suggests that metabolic disorders, including type 2 diabetes mellitus (T2DM), can be treated with traditional Chinese medicine formulas, such as the Gegen Qinlian decoction (GQD). This study elucidates the mechanisms by which gut microbes mediate the anti-diabetic effects of GQD.<h4>Methods</h4>We conducted a double-blind randomized clinical trial involving 120 untreated participants with T2DM. During the 12-week intervention, anthropometric measurements ...[more]

PMID: 38495337

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