C-Src activation as a potential marker of chemical-induced skin irritation using tissue-engineered skin equivalents.

ABSTRACT: Skin irritancy to topically applied chemicals is a significant problem that affects millions of people worldwide. New or modified chemical entities must be tested for potential skin irritancy by industry as part of the safety and toxicity profiling process. Many of these tests have now moved to a non-animal-based format to reduce experiments on animals. However, these tests for irritancy potential often rely on monolayer cultures of keratinocytes that are not representative of the skin architecture or tissue-engineered human skin equivalents (HSE) using complex multi-gene expression panels that are often cumbersome and not amenable for high throughput. Here, we show that human skin equivalents increase abundance of several phosphorylated kinases (c-Src, c-Jun, p53, GSK3α/β) in response to

SUBMITTER: Harding AL 

PROVIDER: S-EPMC10946902 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature