Urinary microbiome-based metagenomic signature for the noninvasive diagnosis of hepatocellular carcinoma.

ABSTRACT:

Background

Gut microbial dysbiosis is implicated in chronic liver disease and hepatocellular carcinoma (HCC), but the role of microbiomes from various body sites remains unexplored. We assessed disease-specific alterations in the urinary microbiome in HCC patients, investigating their potential as diagnostic biomarkers.

Methods

We performed cross-sectional analyses of urine samples from 471 HCC patients and 397 healthy controls and validated the results in an independent cohort of 164 HCC patients and 164 healthy controls. Urinary microbiomes were analyzed by 16S rRNA gene sequencing. A microbial marker-based model distinguishing HCC from controls was built based on logistic regression, and its performance was tested.

Results

Microbial diversity was significantly redu

SUBMITTER: Cho EJ 

PROVIDER: S-EPMC10951239 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature