Use of NanoBiT and NanoBRET to characterise interleukin-23 receptor dimer formation in living cells.

ABSTRACT:

Background and purpose

Interleukin-23 (IL-23) and its receptor are important drug targets for the treatment of auto-inflammatory diseases. IL-23 binds to a receptor complex composed of two single transmembrane spanning proteins IL23R and IL12Rβ1. In this study, we aimed to gain further understanding of how ligand binding induces signalling of IL-23 receptor complexes using the proximity-based techniques of NanoLuc Binary Technology (NanoBiT) and Bioluminescence Resonance Energy Transfer (BRET).

Experimental approach

To monitor the formation of IL-23 receptor complexes, we developed a split luciferase (NanoBiT) assay whereby heteromerisation of receptor subunits can be measured through luminescence. The affinity of NanoBiT complemented complexes for IL-23 was measured using N

SUBMITTER: Lay CS 

PROVIDER: S-EPMC10953408 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature