Dynamic switching of transcriptional regulators between two distinct low-mobility chromatin states.

ABSTRACT: How chromatin dynamics relate to transcriptional activity remains poorly understood. Using single-molecule tracking, coupled with machine learning, we show that histone H2B and multiple chromatin-bound transcriptional regulators display two distinct low-mobility states. Ligand activation results in a marked increase in the propensity of steroid receptors to bind in the lowest-mobility state. Mutational analysis revealed that interactions with chromatin in the lowest-mobility state require an intact DNA binding domain and oligomerization domains. These states are not spatially separated as previously believed, but individual H2B and bound-TF molecules can dynamically switch between them on time scales of seconds. Single bound-TF molecules with different mobilities exhibit different dwell ti

SUBMITTER: Wagh K 

PROVIDER: S-EPMC10954219 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature