Sphingosine induction of the <i>Pseudomonas aeruginosa</i> hemolytic phospholipase C/sphingomyelinase (PlcH).

ABSTRACT: Hemolytic phospholipase C, PlcH, is an important virulence factor for Pseudomonas aeruginosa. PlcH preferentially hydrolyzes sphingomyelin and phosphatidylcholine, and this hydrolysis activity drives tissue damage and inflammation and interferes with the oxidative burst of immune cells. Among other contributors, transcription of plcH was previously shown to be induced by phosphate starvation via PhoB and the choline metabolite, glycine betaine, via GbdR. Here, we show that sphingosine can induce plcH transcription and result in secreted PlcH enzyme activity. This induction is dependent on the sphingosine-sensing transcriptional regulator SphR. The SphR induction of plcH occurs from the promoter for the gene upstream of plcH that encodes the neutral cerami

SUBMITTER: Mackinder JR 

PROVIDER: S-EPMC10955842 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature