Evaluation of Less Invasive Sampling Tools for the Diagnosis of Cutaneous Leishmaniasis.

ABSTRACT:

Background

Diagnosis of cutaneous leishmaniasis (CL) usually relies on invasive samples, but it is unclear whether more patient-friendly tools are good alternatives for diverse lesions when used with polymerase chain reaction (PCR).

Methods

Patients with suspected CL were enrolled consecutively in a prospective diagnostic accuracy study. We compared dental broach, tape disc, and microbiopsy samples with PCR as index tests, using PCR with skin slit samples as reference test. Subsequently, we constructed a composite reference test including microscopy, the 3 index tests and skin slit PCR, and we compared these same tests with the composite reference test. We assessed diagnostic accuracy parameters with 95% confidence intervals for all comparisons.

Results

Among 344 incl

SUBMITTER: van Henten S 

PROVIDER: S-EPMC10977625 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature