Dataset Information

Establishment of porcine fecal-derived ex vivo microbial communities to evaluate the impact of livestock feed on gut microbiome.

ABSTRACT: Sustainable livestock production requires reducing competition for food and feed resources and increasing the utilization of food by-products in livestock feed. This study describes the establishment of an anaerobic batch culture model to simulate pig microbiota and evaluate the effects of a food by-product, wakame seaweed stalks, on ex vivo microbial communities. We selected one of the nine media to support the growth of a bacterial community most similar in composition and diversity to that observed in pig donor feces. Supplementation with wakame altered the microbial profile and short-chain fatty acid composition in the ex vivo model, and a similar trajectory was observed in the in vivo pig experimental validation. Notably, the presence of wakame increased the abundance of Lactobacillus species, which may have been due to cross-feeding with Bacteroides. These results suggest the potential of wakame as a livestock feed capable of modulating the pig microbiome. Collectively, this study highlights the ability to estimate the microbiome changes that occur when pigs are fed a specific feed using an ex vivo culture model.

SUBMITTER: Tsujikawa Y

PROVIDER: S-EPMC10981943 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature

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Establishment of porcine fecal-derived <i>ex vivo</i> microbial communities to evaluate the impact of livestock feed on gut microbiome.

Tsujikawa Yuji Y Nishiyama Keita K Namai Fu F Imamura Yoshiya Y Sakuma Taiga T Saha Sudeb S Suzuki Masahiko M Sakurai Mitsuki M Iwata Ryo R Matsuo Kengo K Takamori Hironori H Suda Yoshihito Y Zhou Binghui B Fukuda Itsuko I Villena Julio J Sakane Iwao I Osawa Ro R Kitazawa Haruki H

Bioscience of microbiota, food and health 20231115 2

Sustainable livestock production requires reducing competition for food and feed resources and increasing the utilization of food by-products in livestock feed. This study describes the establishment of an anaerobic batch culture model to simulate pig microbiota and evaluate the effects of a food by-product, wakame seaweed stalks, on <i>ex vivo</i> microbial communities. We selected one of the nine media to support the growth of a bacterial community most similar in composition and diversity to ...[more]

PMID: 38577893

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Dataset Information

Establishment of porcine fecal-derived ex vivo microbial communities to evaluate the impact of livestock feed on gut microbiome.

Publications

Establishment of porcine fecal-derived <i>ex vivo</i> microbial communities to evaluate the impact of livestock feed on gut microbiome.

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