Biological variation of PIVKA-II in blood serum of healthy subjects measured by automated electrochemiluminescent assay.

ABSTRACT:

Background

Prothrombin/Protein Induced by Vitamin K Absence-II (PIVKA-II) is a candidate biomarker of hepatocellular cancer, recommended both for diagnostics and monitoring. The aim was to evaluate biological variation (BV) of serum PIVKA-II.

Methods

Within-subject (CV_I) and between-subject (CV_G) BV estimates were assessed in 14 healthy volunteers in a 6-week protocol. Serum concentrations of PIVKA-II were measured by a Roche Elecsys PIVKA-II diagnostic kit (cobas e8000). Precision (CV_A) was assessed from duplicate measurements of all volunteers' samples. Two methods were used for the estimation of CV_I: SD-ANOVA and CV-ANOVA method. We calculated the index of individuality (II) and reference change value. The experiment was fully c

SUBMITTER: Jabor A 

PROVIDER: S-EPMC10992686 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature