Pseudo-pac site sequences used by phage P22 in generalized transduction of Salmonella.

ABSTRACT: Salmonella enterica Serovar Typhimurium (Salmonella) and its bacteriophage P22 are a model system for the study of horizontal gene transfer by generalized transduction. Typically, the P22 DNA packaging machinery initiates packaging when a short sequence of DNA, known as the pac site, is recognized on the P22 genome. However, sequences similar to the pac site in the host genome, called pseudo-pac sites, lead to erroneous packaging and subsequent generalized transduction of Salmonella DNA. While the general genomic locations of the Salmonella pseudo-pac sites are known, the sequences themselves have not been determined. We used visualization of P22 sequencing reads mapped to host Salmonella genomes to define regions of generalized transduction initiation an

SUBMITTER: Maier JL 

PROVIDER: S-EPMC10996546 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature