Unknown

Dataset Information

0

Differentiation granules, a dynamic regulator of T. brucei development.


ABSTRACT: Adaptation to a change of environment is an essential process for survival, in particular for parasitic organisms exposed to a wide range of hosts. Such adaptations include rapid control of gene expression through the formation of membraneless organelles composed of poly-A RNA and proteins. The African trypanosome Trypanosoma brucei is exquisitely sensitive to well-defined environmental stimuli that trigger cellular adaptations through differentiation events that characterise its complex life cycle. The parasite has been shown to form stress granules in vitro, and it has been proposed that such a stress response could have been repurposed to enable differentiation and facilitate parasite transmission. Therefore, we explored the composition and positional dynamics of membraneless granules formed in response to starvation stress and during differentiation in the mammalian host between the replicative slender and transmission-adapted stumpy forms. We find that T. brucei differentiation does not reflect the default response to environmental stress. Instead, the developmental response of the parasites involves a specific and programmed hierarchy of membraneless granule assembly, with distinct components and regulation by protein kinases such as TbDYRK, that are required for the parasite to successfully progress through its life cycle development and prepare for transmission.

SUBMITTER: Cayla M 

PROVIDER: S-EPMC10998879 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Differentiation granules, a dynamic regulator of T. brucei development.

Cayla Mathieu M   Spanos Christos C   McWilliam Kirsty K   Waskett Eliza E   Rappsilber Juri J   Matthews Keith R KR  

Nature communications 20240406 1


Adaptation to a change of environment is an essential process for survival, in particular for parasitic organisms exposed to a wide range of hosts. Such adaptations include rapid control of gene expression through the formation of membraneless organelles composed of poly-A RNA and proteins. The African trypanosome Trypanosoma brucei is exquisitely sensitive to well-defined environmental stimuli that trigger cellular adaptations through differentiation events that characterise its complex life cy  ...[more]

Similar Datasets

2024-04-02 | PXD045841 | Pride
| S-EPMC11556693 | biostudies-literature
2016-06-13 | E-MTAB-4557 | biostudies-arrayexpress
| S-EPMC10165536 | biostudies-literature
| S-EPMC5380359 | biostudies-literature
2009-07-10 | GSE17026 | GEO
2013-10-31 | E-GEOD-32991 | biostudies-arrayexpress
| S-EPMC3575708 | biostudies-literature
2010-05-16 | E-GEOD-17026 | biostudies-arrayexpress
| S-EPMC10879817 | biostudies-literature