A Computational Model of Endogenous Hydrogen Peroxide Metabolism in Hepatocytes, Featuring a Critical Role for GSH.

ABSTRACT: This paper presents an ordinary differential equation (ODE) model of endogenous H₂O₂ production and elimination in hepatocytes that is unique, at the time of writing, in its ability to accurately compute intracellular H₂O₂ concentration during incidents of oxidative stress and in its usefulness for constructing PBPK/PD models for ROS-generating xenobiotics. Versions of the model are presented for rat hepatocytes in vitro and mouse liver in vivo. A generic method is given for using the model to create PBPK/PD models which predict intracellular H₂O₂ concentration and oxidative-stress-induced hepatocyte death; these are identifiable from in vitro data sets reporting cell mortality following xenobiotic expos

SUBMITTER: Bilinsky LM 

PROVIDER: S-EPMC11044893 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature