Thermostable vacuum foam dried Newcastle disease vaccine: Process optimization and pilot-scale study.

ABSTRACT: Vacuum foam drying (VFD) has been shown to improve the thermostability and long-term shelf life of Newcastle Disease Virus (NDV). This study optimized the VFD process to improve the shelf life of NDV at laboratory-scale and then tested the optimized conditions at pilot-scale. The optimal NDV to T5 formulation ratio was determined to be 1:1 or 3:2. Using the 1:1 virus to formulation ratio, the optimal filling volumes were determined to be 13-17% of the vial capacity. The optimized VFD process conditions were determined to be at a shelf temperature of 25℃ with a minimum overall drying time of 44 h. The vaccine samples prepared using these optimized conditions at laboratory-scale exhibited virus titer losses of ≤ 1.0 log₁₀ with residual moisture content (RMC) below 3%. Furthermore,

SUBMITTER: Lyu F 

PROVIDER: S-EPMC11153293 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature