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The pioneer transcription factor Zelda facilitates the exit from regeneration and restoration of patterning in Drosophila.


ABSTRACT: For a damaged tissue to regenerate, the injured site must repair the wound, proliferate, and restore the correct patterning and cell types. We found that Zelda, a pioneer transcription factor largely known for its role in embryonic zygotic genome activation, is dispensable for normal wing development but crucial for wing disc patterning during regeneration. Impairing Zelda function during disc regeneration resulted in adult wings with a plethora of cell fate errors, affecting the veins, margins, and posterior compartment identity. Using CUT&RUN, we identified and validated targets of Zelda including the cell fate genes cut, Delta and achaete, which failed to return to their normal expression patterns upon loss of Zelda. In addition, Zelda controls expression of factors previously established to preserve cell fate during regeneration like taranis and osa, which stabilizes engrailed expression during regeneration, thereby preserving posterior identity. Finally, Zelda ensures proper expression of the integrins encoded by multiple edematous wings and myospheroid during regeneration to prevent blisters in the resuting adult wing. Thus, Zelda is crucial for maintaining cell fate and structural architecture of the regenerating tissue.

SUBMITTER: Bose A 

PROVIDER: S-EPMC11160785 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature

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The pioneer transcription factor Zelda facilitates the exit from regeneration and restoration of patterning in <i>Drosophila</i>.

Bose Anish A   Schuster Keaton K   Kodali Chandril C   Sonam Surabhi S   Smith-Bolton Rachel R  

bioRxiv : the preprint server for biology 20240530


For a damaged tissue to regenerate, the injured site must repair the wound, proliferate, and restore the correct patterning and cell types. We found that Zelda, a pioneer transcription factor largely known for its role in embryonic zygotic genome activation, is dispensable for normal wing development but crucial for wing disc patterning during regeneration. Impairing Zelda function during disc regeneration resulted in adult wings with a plethora of cell fate errors, affecting the veins, margins,  ...[more]

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