The pioneer transcription factor Zelda facilitates the exit from regeneration and restoration of patterning in <i>Drosophila</i>.

ABSTRACT: For a damaged tissue to regenerate, the injured site must repair the wound, proliferate, and restore the correct patterning and cell types. We found that Zelda, a pioneer transcription factor largely known for its role in embryonic zygotic genome activation, is dispensable for normal wing development but crucial for wing disc patterning during regeneration. Impairing Zelda function during disc regeneration resulted in adult wings with a plethora of cell fate errors, affecting the veins, margins, and posterior compartment identity. Using CUT&RUN, we identified and validated targets of Zelda including the cell fate genes cut, Delta and achaete, which failed to return to their normal expression patterns upon loss of Zelda. In addition, Zelda controls expression of factors previo

SUBMITTER: Bose A 

PROVIDER: S-EPMC11160785 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature