Deletion of a core APC/C component reveals APC/C function in regulating neuronal USP1 levels and morphology.

ABSTRACT:

Introduction

The Anaphase Promoting Complex (APC/C), an E3 ubiquitin ligase, plays a key role in cell cycle control, but it is also thought to operate in postmitotic neurons. Most studies linking APC/C function to neuron biology employed perturbations of the APC/C activators, cell division cycle protein 20 (Cdc20) and Cdc20 homologue 1 (Cdh1). However, multiple lines of evidence indicate that Cdh1 and Cdc20 can function in APC/C-independent contexts, so that the effects of their perturbation cannot strictly be linked to APC/C function.

Methods

We therefore deleted the gene encoding Anaphase Promoting Complex 4 (APC4), a core APC/C component, in neurons cultured from conditional knockout (cKO) mice.

Results

Our data indicate that several previously published substrates

SUBMITTER: Day JL 

PROVIDER: S-EPMC11199872 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature