MiPEP31 alleviates Ang II-induced hypertension in mice by occupying Cebpα binding sites in the pri-miR-31 promoter.

ABSTRACT:

Background

Previous studies have shown that peptides encoded by noncoding RNAs (ncRNAs) can be used as peptide drugs to alleviate diseases. We found that microRNA-31 (miR-31) is involved in the regulation of hypertension and that the peptide miPEP31, which is encoded by the primary transcript of miR-31 (pri-miR-31), can inhibit miR-31 expression. However, the role and mechanism of miPEP31 in hypertension have not been elucidated.

Methods

miPEP31 expression was determined by western blot analysis. miPEP31-deficient mice (miPEP31^-/-) were used, and synthetic miPEP31 was injected into Ang II-induced hypertensive mice. Blood pressure was monitored through the tail-cuff method. Histological staining was used to evaluate renal damage. Regulatory T (T_reg) cell

SUBMITTER: Li X 

PROVIDER: S-EPMC11241881 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature