The <i>Staphylococcus aureus</i> small non-coding RNA IsrR regulates TCA cycle activity and virulence.

ABSTRACT: Staphylococcus aureus has evolved mechanisms to cope with low iron (Fe) availability in host tissues. S. aureus uses the ferric uptake transcriptional regulator (Fur) to sense titers of cytosolic Fe. Upon Fe depletion, apo-Fur relieves transcriptional repression of genes utilized for Fe uptake. We demonstrate that an S. aureus Δfur mutant has decreased expression of acnA, which codes for the Fe-dependent enzyme aconitase. Decreased acnA expression prevented the Δfur mutant from growing with amino acids as sole carbon and energy sources. Suppressor analysis determined that a mutation in isrR, which produces a regulatory RNA, permitted growth by decreasing isrR transcription. The decreased AcnA activity of the Δfur mutant

SUBMITTER: Rios-Delgado G 

PROVIDER: S-EPMC11245030 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature