Activation of Nrf2 inhibits atherosclerosis in ApoE^-/- mice through suppressing endothelial cell inflammation and lipid peroxidation.

ABSTRACT:

Background

Nuclear erythroid 2-related factor 2 (Nrf2), a transcription factor, is critically involved in the regulation of oxidative stress and inflammation. However, the role of endothelial Nrf2 in atherogenesis has yet to be defined. In addition, how endothelial Nrf2 is activated and whether Nrf2 can be targeted for the prevention and treatment of atherosclerosis is not explored.

Methods

RNA-sequencing and single-cell RNA sequencing analysis of mouse atherosclerotic aortas were used to identify the differentially expressed genes. In vivo endothelial cell (EC)-specific activation of Nrf2 was achieved by injecting adeno-associated viruses into ApoE^-/- mice, while EC-specific knockdown of Nrf2 was generated in Cdh5^CreCas9^floxed-stopApoE^-

SUBMITTER: He L 

PROVIDER: S-EPMC11247299 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature