Evaluation of thrombomodulin/thrombin activatable fibrinolysis inhibitor function in plasma using tissue-type plasminogen activator-induced plasma clot lysis time.

ABSTRACT:

Background

Thrombin activatable fibrinolysis inhibitor (TAFI) is one of the most important physiological fibrinolysis inhibitors. Its inhibitory efficacy under physiological conditions remains uncertain.

Objectives

Elucidate the role of soluble thrombomodulin (sTM)/TAFI axis in the regulation of fibrinlysis.

Methods

Since thrombin is required to generate activated TAFI (TAFIa) that targets the C-terminal lysine of partially digested fibrin, a clot lysis assay is suitable for evaluating its function. Using tissue-type plasminogen activator-induced plasma clot lysis time (tPA-PCLT) together with TAFIa inhibitor and recombinant sTM (rsTM), we evaluated the specific function of TM/TAFI in the plasma milieu.

Results

tPA-PCLT values were significantly shortened by th

SUBMITTER: Urano T 

PROVIDER: S-EPMC11255936 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature