A Cost-effective, High-throughput, Highly Accurate Genotyping Method for Outbred Populations.

ABSTRACT: Affordable sequencing and genotyping methods are essential for large scale genome-wide association studies. While genotyping microarrays and reference panels for imputation are available for human subjects, non-human model systems often lack such options. Our lab previously demonstrated an efficient and cost-effective method to genotype heterogeneous stock rats using double-digest genotyping-by-sequencing. However, low-coverage whole-genome sequencing offers an alternative method that has several advantages. Here, we describe a cost-effective, high-throughput, high-accuracy genotyping method for N/NIH heterogeneous stock rats that can use a combination of sequencing data previously generated by double-digest genotyping-by-sequencing and more recently generated by low-coverage whole-genome-

SUBMITTER: Chen D 

PROVIDER: S-EPMC11275765 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature