Upregulation of TET2 and Resistance to DNA Methyltransferase (DNMT) Inhibitors in DNMT1-Deleted Cancer Cells.

ABSTRACT:

Background

Ten-eleven-translocation (TET) 2 is a member of the TET family of proteins (TET1-3). DNMT1 gene deletion confers resistance to DNA methyltransferase (DNMT) inhibitors in colorectal, breast, and ovarian cancer cells. Currently, the effect of DNMT1 gene status on TET2 phenotype following DNMT inhibitor treatment is unclear in human malignancies.

Methods

Human colorectal carcinoma HCT116 cells (DNMT^+/+) and their isogenic DNMT1 knockout (DNMT1^-/-) counterpart were treated with DNMT inhibitors. Expression of TET2 and tumor suppressor (p16^ink4A and p15^ink4B) proteins were examined by Western blot. Apoptosis and CDKN2A promoter demethylation following drug treatment were detected by Annexin-V

SUBMITTER: Laranjeira ABA 

PROVIDER: S-EPMC11276550 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature