Deciphering the interplay of HPV infection, MHC-II expression, and CXCL13⁺ CD4⁺ T cell activation in oropharyngeal cancer: implications for immunotherapy.

ABSTRACT:

Background

Human papillomavirus (HPV) infection has become an important etiological driver of oropharyngeal squamous cell carcinoma (OPSCC), leading to unique tumor characteristics. However, the interplay between HPV-associated tumor cells and tumor microenvironment (TME) remains an enigma.

Methods

We performed a single-cell RNA-sequencing (scRNA-seq) on HPV-positive (HPV⁺) and HPV-negative (HPV^‒) OPSCC tumors, each for three samples, and one normal tonsil tissue. Ex vivo validation assays including immunofluorescence staining, cell line co-culture, and flow cytometry analysis were used to test specific subtypes of HPV⁺ tumor cells and their communications with T cells.

Results

Through a comprehensive single-cell transcriptome analysis

SUBMITTER: Yan S 

PROVIDER: S-EPMC11303625 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature