Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol.

ABSTRACT: Virus-Induced Gene Silencing (VIGS) is a versatile tool in plant science, yet its application to non-model species like sunflower demands extensive optimization due to transformation challenges. In this study, we aimed to elucidate the factors that significantly affect the efficiency of Agrobacterium-VIGS in sunflowers. After testing a number of approaches, we concluded that the seed vacuum technique followed by 6 h of co-cultivation produced the most efficient VIGS results. Genotype-dependency analysis revealed varying infection percentages (62-91%) and silencing symptom spreading in different sunflower genotypes. Additionally, we explored the mobility of tobacco rattle virus (TRV) and phenotypic silencing manifestation (photo-bleaching) across different tissues and regions of VIGS-infect

SUBMITTER: Mardini M 

PROVIDER: S-EPMC11318282 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature