Downregulation of GPX8 in hepatocellular carcinoma: impact on tumor stemness and migration.

ABSTRACT:

Purpose

GPX8, which is found in the endoplasmic reticulum lumen, is a member of the Glutathione Peroxidases (GPXs) family. Its role in hepatocellular carcinoma (HCC) is unknown.

Methods

Immunohistochemical staining was used to detect the protein levels of GPX8 in HCC tissue microarrays. A short hairpin RNA lentivirus was used to knock down GPX8, and the main signaling pathways were investigated using transcriptome sequencing and a phosphorylated kinase array. The sphere formation assays, cloning-formation assays and cell migration assays were used to evaluate the stemness and migration ability of HCC cells. Identifying the GPX8-interacting proteins was accomplished through immunoprecipitation and protein mass spectrometry.

Results

The GPX8 protein levels were downregu

SUBMITTER: Tao CY 

PROVIDER: S-EPMC11322209 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature