Quadrant darkfield (QDF) for label-free imaging of intracellular puncta.

ABSTRACT:

Significance

Measuring changes in cellular structure and organelles is crucial for understanding disease progression and cellular responses to treatments. A label-free imaging method can aid in advancing biomedical research and therapeutic strategies.

Aim

This study introduces a computational darkfield imaging approach named quadrant darkfield (QDF) to separate smaller cellular features from large structures, enabling label-free imaging of cell organelles and structures in living cells.

Approach

Using a programmable LED array as illumination source, we vary the direction of illumination to encode additional information about the feature size within cells. This is possible due to the varying level of directional scattering produced by features based on their sizes rela

SUBMITTER: Moustafa TE 

PROVIDER: S-EPMC11326191 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature