NXPH4 mediated by m⁵C contributes to the malignant characteristics of colorectal cancer via inhibiting HIF1A degradation.

ABSTRACT:

Objective

Colorectal cancer (CRC) is a form of malignancy that exhibits a comparatively elevated occurrence and fatality rate. Given the relatively slower progress in diagnostic and therapeutic approaches for CRC, there is a need to investigate more accurate and efficient biomarkers.

Methods

Core regulatory genes were screened using the TCGA database, and the expression of neurexophilin 4 (NXPH4) and its prognostic implications were validated using tissue microarray staining. The assessment of NXPH4 functions involved a range of experiments, including cellular, organoid, and murine models. Furthermore, a regulatory network between m⁵C, NXPH4, and HIF1A was established through several in vitro experiments.

Results

The overexpression of NXPH4 is associated wi

SUBMITTER: Yang L 

PROVIDER: S-EPMC11334498 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature