Fusiform nanoparticle boosts efficient genetic transformation in Sclerotinia sclerotiorum.

ABSTRACT:

Background

Sclerotinia sclerotiorum is a highly destructive phytopathogenic fungus that poses a significant threat to a wide array of crops. The current constraints in genetic manipulation techniques impede a thorough comprehension of its pathogenic mechanisms and the development of effective control strategies.

Results

Herein, we present a highly efficient genetic transformation system for S. sclerotiorum, leveraging the use of fusiform nanoparticles, which are synthesized with FeCl₃ and 2,6-diaminopyrimidine (DAP). These nanoparticles, with an average longitude length of 59.00 nm and a positively charged surface, facilitate the direct delivery of exogenous DNA into the mycelial cells of S. sclerotiorum, as well as successful integration with stable expression. N

SUBMITTER: Ding Y 

PROVIDER: S-EPMC11334516 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature