TF-High-Evolutionary: In Vivo Mutagenesis of Gene Regulatory Networks for the Study of the Genetics and Evolution of the Drosophila Regulatory Genome.
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ABSTRACT:
SUBMITTER: Li XC
PROVIDER: S-EPMC11342961 | biostudies-literature | 2024 Aug
REPOSITORIES: biostudies-literature
Publications
TF-High-Evolutionary: In Vivo Mutagenesis of Gene Regulatory Networks for the Study of the Genetics and Evolution of the Drosophila Regulatory Genome.
Li Xueying C XC Srinivasan Vani V Laiker Ian I Misunou Natalia N Frankel Nicolás N Pallares Luisa F LF Crocker Justin J
Molecular biology and evolution 20240801 8
Understanding the evolutionary potential of mutations in gene regulatory networks is essential to furthering the study of evolution and development. However, in multicellular systems, genetic manipulation of regulatory networks in a targeted and high-throughput way remains challenging. In this study, we designed TF-High-Evolutionary (HighEvo), a transcription factor (TF) fused with a base editor (activation-induced deaminase), to continuously induce germline mutations at TF-binding sites across ...[more]
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