Comparative genomics reveal unique markers to monitor by routine PCR assay bioinoculant of <i>Sphingobium indicum</i> B90A in hexachlorocyclohexane (HCH) contaminated soils.

ABSTRACT: Bioinoculants of Sphingobium indicum B90A have been used to decontaminate hexachlorocyclohexane (HCH)-contaminated soils in the past. There is no selective or convenient method available to track the added B90A in HCH-contaminated soils in the presence of several native sphingomonads. Here, we describe a method, BioMarkTrack, for tracking B90A bioinoculant by simple amplification of the B90A specific biomarker genes. Whole-genome sequence data of 120 different genera of sphingomonads (Sphingobium, Novosphingobium, Sphingomonas, Sphingopyxis, and Sphingosinicella) were retrieved from the NCBI database and annotated. Intra- and inter-genus similarity searches, including the genome of B90A as a reference was conducted. 122 unique gene sequences were i

SUBMITTER: Phian S 

PROVIDER: S-EPMC11399485 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature