Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii.

ABSTRACT:

Background

The β-galactosidase from Paenibacillus wynnii (β-gal-Pw) is a promising candidate for lactose hydrolysis in milk and dairy products, as it has a higher affinity for the substrate lactose (low K_M value) compared to industrially used β-galactosidases and is not inhibited by the hydrolysis-generated product D-galactose. However, β-gal-Pw must firstly be produced cost-effectively for any potential industrial application. Accordingly, the yeast Komagataella phaffii was chosen to investigate its feasibility to recombinantly produce β-gal-Pw since it is approved for the regulated production of food enzymes. The aim of this study was to find the most suitable way to produce the β-gal-Pw in K. phaffii either extracellularly or intracellularly.

Results

Firstly, 1

SUBMITTER: Bechtel A 

PROVIDER: S-EPMC11452983 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature