EMC1 Is Required for the Sarcoplasmic Reticulum and Mitochondrial Functions in the <i>Drosophila</i> Muscle.

ABSTRACT: EMC1 is part of the endoplasmic reticulum (ER) membrane protein complex, whose functions include the insertion of transmembrane proteins into the ER membrane, ER-mitochondria contact, and lipid exchange. Here, we show that the Drosophila melanogaster EMC1 gene is expressed in the somatic musculature and the protein localizes to the sarcoplasmic reticulum (SR) network. Muscle-specific EMC1 RNAi led to severe motility defects and partial late pupae/early adulthood lethality, phenotypes that are rescued by co-expression with an EMC1 transgene. Motility impairment in EMC1-depleted flies was associated with aberrations in muscle morphology in embryos, larvae, and adults, including tortuous and misaligned fibers with reduced size and weakness. They were also associated with

SUBMITTER: Couto-Lima CA 

PROVIDER: S-EPMC11506464 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature