Evaluation of general anesthesia protocols for a highly controlled cardiac ischemia-reperfusion model in mice.

ABSTRACT:

Background

The aim of our study was to test different anesthetic mixtures in order to identify the most suitable one for a surgical cardiac ischemia-reperfusion model in mice.

Methods

1) Sixty four mice were submitted to one of the 6 combinations of ketamine or alfaxalone associated to xylazine, medetomidine or midazolam. Depth and quality of anesthesia were evaluated via 5 reflex scores. 2) Impact of analgesic (buprenorphine or butorphanol), anesthesia reversal (with atipamezole) and surgery (cardiac ischemia-reperfusion surgery) have been tested in the selected protocols. 3) infarction size has been measured with TTC (Triphenyl Tetrazolium Chloride) method in mice anesthetized with best protocols.

Results

Protocol involving medetomidine induced the longest surgical

SUBMITTER: Leon C 

PROVIDER: S-EPMC11508169 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature