Investigating Flavonoids by HPTLC Analysis Using Aluminium Chloride as Derivatization Reagent.

ABSTRACT: This is the first study to report on high performance thin layer chromatography (HPTLC) generated spectrophotometric data to systematically capture flavonoid compounds using optimized derivatization with either AlCl₃ or NaNO₂-AlCl₃-NaOH as visualisation reagents. While the traditional AlCl₃ colorimetric method using UV-Vis analysis provides valuable insights into the presence of flavonoids and allows derivation of the total flavonoid content (TFC) of a sample, HPTLC fingerprints obtained after spraying with AlCl₃ or NaNO₂-AlCl₃-NaOH enable the visualization of the various flavonoids present in a sample based on their respective absorption shifts, thus complementing the traditional TFC assay. In this study, 40 diff

SUBMITTER: Sultana S 

PROVIDER: S-EPMC11547264 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature