A coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores.

ABSTRACT: Cells coordinate diverse events at anaphase onset, including separase activation, cohesin cleavage, chromosome separation, and spindle reorganization. Regulation of the XMAP215 family member and microtubule polymerase, Stu2, at the metaphase-anaphase transition determines a specific redistribution from kinetochores to spindle microtubules. We show that cells modulate Stu2 kinetochore-microtubule localization by Polo-like kinase1/Cdc5-mediated phosphorylation of T866, near the Stu2 C-terminus, thereby promoting dissociation from the kinetochore Ndc80 complex. Cdk/Cdc28 likely primes Cdc5:Stu2 interaction. Cdc28 activity is also required for Stu2 nuclear import. PP2A^Cdc55 actively opposes Cdc5 activity on Stu2^T866 during metaphase. This counter-regulation allows for swi

SUBMITTER: Stewart MG 

PROVIDER: S-EPMC11566010 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature