Ligand binding characteristics of an NAD+ riboswitch revealed by FRET and biolayer interferometry.

ABSTRACT: The Class II NAD⁺ riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with β-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg²⁺.

SUBMITTER: Conoan Nieves NE 

PROVIDER: S-EPMC11601409 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature