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Ligand binding characteristics of an NAD+ riboswitch revealed by FRET and biolayer interferometry.

ABSTRACT: The Class II NAD+ riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with β-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg2+.

SUBMITTER: Conoan Nieves NE 

PROVIDER: S-EPMC11601409 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature

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Ligand binding characteristics of an NAD+ riboswitch revealed by FRET and biolayer interferometry.

Conoan Nieves Nico E NE   Widom Julia R JR  

bioRxiv : the preprint server for biology 20241111

The Class II NAD<sup>+</sup> riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with β-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg<sup>2+</sup>. ...[more]

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