High-level production of γ-aminobutyric acid via efficient co-expression of the key genes of glutamate decarboxylase system in <i>Escherichia coli</i>.

ABSTRACT: Biosynthesis of the functional factor γ-aminobutyric acid (GABA) in bacteria involves two key proteins an intracellular glutamate decarboxylase (GadB) and a membrane-bound antiporter (GadC). Efficient co-expression of suitable GadB and GadC candidates is crucial for improving GABA productivity. In this study, gadB _ΔC11 of Lactiplantibacillus plantarum and gadC _ΔC41 of Escherichia coli were inserted into the designed double promoter (P _T7lac and P _BAD ) expression system. Then, E. coli Lemo21(DE3) was chosen as the host to minimize the toxic effects of GadC_ΔC41 overexpression. Furthermore, a green and high-efficiency GABA synthesis system using dormant engineered Lemo21(DE3) cells as bioc

SUBMITTER: Yao L 

PROVIDER: S-EPMC11610993 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature