Integrated DNA estimation in tissue biopsy and detection in liquid biopsy by HBV-targeted NGS assay.

ABSTRACT:

Background & aims

Integrated HBV DNA (iDNA) plays a critical role in HBV pathogenesis, particularly in predicting treatment response and HCC. This study aimed to use an HBV hybridization-capture next-generation sequencing (HBV-NGS) assay to detect HBV-host junction sequences (HBV-JS) in a sensitive nonbiased manner to detect and estimate the iDNA fraction in tissue biopsies and HBV genetics by liquid biopsy.

Methods

HBV DNA from plasmid monomers, HBV-HCC cell line (SNU398, Hep3B, and PLC/PRF/5), tissue biopsies of patients with serum HBV DNA <4 log IU/ml, and matched urine and plasma of HBV patients were assessed by HBV-NGS. Junction-specific qPCR (JS-qPCR) assays were developed to quantify abundant HBV-JS.

Results

We demonstrated high coverage uniformity, reproducibi

SUBMITTER: Liu HN 

PROVIDER: S-EPMC11643158 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature