T cell induced expression of Coronin-1A facilitates blood-brain barrier transmigration of breast cancer cells.

ABSTRACT: In previous work we discovered that T lymphocytes play a prominent role in the rise of brain metastases of ER-negative breast cancers. In the present study we explored how T lymphocytes promote breast cancer cell penetration through the blood brain barrier (BBB). An in vitro BBB model was employed to study the effects of T lymphocytes on BBB trespassing capacity of three different breast carcinoma cell lines. Differential protein expression was explored by comparing the proteomes of the breast cancer cells before and after co-culture with activated T lymphocytes using liquid chromatography-mass spectrometry (LC-MS). siRNA was used to silence protein expression in the breast cancer cells to study contribution to in vitro BBB passage. Furthermore, protein expression in primary breast cancer

SUBMITTER: Kros JM 

PROVIDER: S-EPMC11682172 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature